Antineoplastic Effects of α-Santalol on Estrogen Receptor-Positive and Estrogen Receptor-Negative Breast Cancer Cells through Cell Cycle Arrest at G2/M Phase and Induction of Apoptosis
Figure 5
α-Santalol induces activation of different caspases and PARP cleavage in MCF-7 cells.
Total cell lysates were prepared from the cells treated with the indicated concentration of α-sanatlol for 12 and 24 h. Equal amounts of proteins were separated by SDS-PAGE and subjected to western immunoblotting. Membranes were probed with respective primary antibodies followed by appropriate secondary antibody and protein expression was determined by ECL detection system. β-actin was used to verify equal loading of the samples.