Targeting CDH17 Suppresses Tumor Progression in Gastric Cancer by Downregulating Wnt/β-Catenin Signaling
Figure 5
CDH17 activates multiple signal transduction pathways.
A. Expression of β-catenin, GSK-3β, p- GSK-3β, Rb, Cyclin D1were compared between shCDH17 and Mock both in AGS and MKN-45 cells by Western blot analysis. MDM2, p53 and p21 also were evaluated. β-Actin was used as a loading control. B. TOPflash/FOPflash reporter assay shows that Wnt signaling re-activated after CDH17 restoration in AGS and MKN-45. (* P<0.05). C. Increase of both the total and nuclear β-catenin protein after restoration of CDH17 in AGS and MKN-45, when compared with the shCDH17.