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Regulation of the Membrane Insertion and Conductance Activity of the Metamorphic Chloride Intracellular Channel Protein CLIC1 by Cholesterol

Figure 2

Membrane Conductance of Lysteriolsin.

Representative impedance spectroscopy recordings of (A-E) 2 µM Lysteriolysin and (F) buffer control, added to tethered membranes made from AM199 containing varying amounts of cholesterol (mol% chol) (A) 50, (B) 25, (C) 12.5, (D) 6.25, (E) 0, (F) 50; [n = 3].

Figure 2

doi: https://doi.org/10.1371/journal.pone.0056948.g002