The Sclerotinia sclerotiorum Mating Type Locus (MAT) Contains a 3.6-kb Region That Is Inverted in Every Meiotic Generation
Figure 5
Proposed MAT1-1 – MAT1-2 border in S. sclerotiorum MAT inferred from an alignment of S. sclerotiorum MAT, B. cinerea MAT1-1 and MAT1-2.
The location of the alignment reproduced above is indicated in Figure 4 by an asterisk, for entire alignment see Alignment S3. Sclerotinia sclerotiorum strain 44Ba1 MAT (GenBank Accession JQ815883) and B. cinerea MAT1-1 (AAID01003685) were alignable up to 248 bp downstream of S. sclerotiorum MAT1-1-1 as indicated by the top left arrow, homology ceases thereafter. The site where a crossover between ancestral MAT1-1 and MAT1-2 represented here by B. cinerea MAT1-1 and MAT1-2, might have occurred, is underlined. The border and crossover site positions are tentative, since S. sclerotiorum MAT and B. cinerea MAT1-2 (reverse complement of FQ790352) were too divergent to be aligned in the MAT1-2-4 downstream region (Figure 1), the top right arrow demarcates the MAT1-2 region in S. sclerotiorum based on lack of homology to B. cinerea MAT1-1, not based on homology to B. cinerea MAT1-2.