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Warburg and Crabtree Effects in Premalignant Barrett's Esophagus Cell Lines with Active Mitochondria

Figure 2

CP-D displays a greater OCR and ECAR response to glycolytic inhibition than other cell lines.

Following addition of 50 mM 2-DG, total (a) ECAR and (b) OCR were measured on the Seahorse XF24 analyzer for each cell line and changes versus untreated baseline are plotted. Error bars represent standard-deviation of means between experiments (N = 2–4). Each experiment consisted of 3–4 replicate wells per cell line with four serial measures performed on each well. p-values (Tukey-Kramer test) of statistically significant differences from CP-A are shown.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0056884.g002