Inhibition of Pirfenidone on TGF-beta2 Induced Proliferation, Migration and Epithlial-Mesenchymal Transition of Human Lens Epithelial Cells Line SRA01/04
Figure 5
Inhibitory effect of pirfenidone on TGF-β2-induced epithlial-mesenchymal transition in SRA01/04 cells.
Compared with control cells (A), immunocytofluorescence demonstrated that the TGF-beta2 significantly up-regulated the mesenchymal phenotypic marker fibronectin (FN, green) in SRA01/04 cells (C). Either in the absence (B) or presence (D) of TGF-beta2, FN was significantly down-regulated after 24 hours’ treatment with 0.5 mg/ml PFD. The nuclei stained by DAPI (blue). Magnification, ×400.