TWEAK Enhances E-selectin and ICAM-1 Expression, and May Contribute to the Development of Cutaneous Vasculitis
Figure 10
SB203580 and SP600125 but not PD98059 block TWEAK-induced adhesion molecules protein expression.
Cells were pre-treated with various MAPKs inhibitors (SB203580, SP600125 and PD98059) for 0.5 h followed by incubation with TWEAK 100 ng/ml for a further 6 h. After treatment, cells were stained with FITC-labeled antibodies against E-selectin and ICAM-1. The expression of E-selectin (A and B) and ICAM-1 (A and C) were quantitatively analyzed by flow cytometry and depicted in the dark grey histograms. Cells stained with FITC-labeled IC mAb were depicted in the light grey histograms. Values are expressed as Mean ± SD; one-way analysis of variance n = 4. **P<0.01, compared with untreated group; ##P<0.01, compared with TWEAK 100 ng/ml-treated group.