TWEAK Enhances E-selectin and ICAM-1 Expression, and May Contribute to the Development of Cutaneous Vasculitis
Figure 8
TWEAK induces the adhesion of human PMNs to HDMECs.
Cells were treated with TWEAK (1–100 ng/ml), TNF-a 10 ng/ml or TWEAK 100 ng/ml plus anti-human Fn14 mAb or IC mAb for 6 h, and then co-incubated with fluorescent-labeled PMNs for 0.5 h. After treatment, PMNs bound to HDMECs were lysed. Fluorescent intensities of cell lysate were counted on a spectrofluorometer. Values are expressed as Mean ± SD; one-way analysis of variance n = 4. *P<0.05, **P<0.01, compared with untreated group; ##P<0.01, compared with TWEAK 100 ng/ml-treated group.