TWEAK Enhances E-selectin and ICAM-1 Expression, and May Contribute to the Development of Cutaneous Vasculitis
Figure 7
TWEAK enhances adhesion molecule expression in HDMECs.
Cells were treated with TWEAK (1–100 ng/ml), TNF-a 10 ng/ml, TWEAK 100 ng/ml plus anti-human Fn14 mAb or IC mAb for 6 h. After treatment, cells were stained with FITC-labeled antibodies against E-selectin and ICAM-1. The expression of E-selectin (A and C) and ICAM-1 (B and D) were quantitatively analyzed by flow cytometry and depicted in the dark grey histograms. Cells stained with FITC-labeled IC mAb were depicted in the light grey histograms. Values are expressed as Mean ± SD; one-way analysis of variance n = 4.*P<0.05, **P<0.01, compared with untreated group; ##P<0.01, compared with TWEAK 100 ng/ml-treated group.