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TWEAK Enhances E-selectin and ICAM-1 Expression, and May Contribute to the Development of Cutaneous Vasculitis

Figure 4

TWEAK priming upregulates but TWEAK blockade suppresses adhesion molecules expression and MPO activity.

Mice were treated as described above, tissue sample were collected 6 h after systemic LPS challenge (six mice per group). Skin sections were stained with antibodies against E-selectin (A) and ICAM-1 (B). These observations were representative of multiple experiments and microscopic fields. Scale bars, 10 µm. (C) MPO activity was assessed for the quantification of PMNs accumulation in tissue samples by a colorimetric assay. Values are expressed as Mean ± SD; Mann–Whitney U-test **P<0.01.

Figure 4

doi: https://doi.org/10.1371/journal.pone.0056830.g004