TWEAK Enhances E-selectin and ICAM-1 Expression, and May Contribute to the Development of Cutaneous Vasculitis
Figure 3
TWEAK neutralization blocks LPS induced CV.
Mice were treated as described (six mice per group). (A) 6 h after systemic LPS challenge, the representative macroscopic appearance of the hemorrhagic lesions in the ears was shown. Tissue samples were collected and stained for hematoxylin–eosin (B) and Masson's trichrome (C). Scale bars, 50 µm. (D) The hemorrhagic lesions in different group were determined by a semiquantitative score as described 21 at the indicated time points. (E) Extravasation of FITC-BSA was assessed spectrophotometrically in the ear from CV mice treated with anti-TWEAK mAb or IC mAb or PBS-treated mice (control group). Values are expressed as Mean ± SD; Mann–Whitney U-test *P<0.05, **P<0.01, compared with IC mAb-treated group; ##P<0.01, compared with control group.