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TWEAK Enhances E-selectin and ICAM-1 Expression, and May Contribute to the Development of Cutaneous Vasculitis

Figure 2

TWEAK priming induces a local CV.

Mice were treated as described (six mice per group). (A) 6 h after LPS challenge, the hemorrhagic lesions in the ears were observed in TWEAK priming group. Tissue samples were stained for hematoxylin–eosin (B) and Masson's trichrome (C). Representative images were shown from three experiments. Scale bars, 50 µm. (D) The number and size of hemorrhagic lesions were analyzed according to the semiquantitative score as described 21 at different time points. (E) Increased FITC-BSA extravasation was found in the left ear from TWEAK priming group and LPS priming group. Values are expressed as Mean ± SD; Mann–Whitney U-test *P<0.05, **P<0.01, compared with PBS priming group; ##P<0.01, compared with TWEAK priming group.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0056830.g002