The SWI/SNF Chromatin Remodeling Complex Influences Transcription by RNA Polymerase I in Saccharomyces cerevisiae
Figure 5
A small accumulation of Pol I complexes in the 5′ end of rDNA in snf6Δ.
(A) Distribution frequency for the number of polymerases per gene was revealed by EM analysis of Miller chromatin spreads in snf6Δ (DAS647) and WT (DAS648). (B) More than 100 rDNA genes from Miller chromatin spreads were analyzed in WT and snf6Δ. Pol I density and percentage of actively transcribed genes in snf6Δ and WT are similar. (C) Polymerase occupancy as a function of position within the transcribed region of rDNA in snf6Δ and WT cells. A small peak of Pol I occupancy in the 5′ end of rDNA in snf6Δ is indicated by an asterisk. All mappable genes in the dataset were analyzed, corresponding to >60 genes per strain and >2500 polymerases per strain. Schematic below the X-axis represents the Pol I transcribed region of the rDNA. (D) Same as C but an additional two WT control strains (NOY388 and BY4741) are plotted. BY4741 data are from El Hage et al. 2010 [34].