Induction of Apoptosis in Human Breast Cancer Cells via Caspase Pathway by Vernodalin Isolated from Centratherum anthelminticum (L.) Seeds
Figure 12
Effect of vernodalin on nuclear morphology, membrane permeabilization, MMP (Δψm) and cytochrome c release.
MCF-7 or MDA-MB-231 cells were plated in 96-well plates and treated with either vehicle (DMSO) or indicated dosages of vernodalin for 24 hours. Cells were fixed and stained according to the manual. Images were acquired using Cellomic HCS array scan reader (objective 20 ×). (A) Representative figures showing changes in DNA content (blue), cell permeability (green), MMP (red) and cytochrome c (cyan). Arrows showed condensed or fragmented DNA. (B) Bar chart showing dose-dependent increased in cell permeability, reduced MMP and increased cytochrome c release in vernodalin-treated samples. Data were mean ± SD of fluorescence intensity readings of three independent experiments. (*P<0.05).