Induction of Apoptosis in Human Breast Cancer Cells via Caspase Pathway by Vernodalin Isolated from Centratherum anthelminticum (L.) Seeds
Figure 11
Vernodalin mediates ROS production.
(A) MCF-7 or MDA-MB-231 cells were treated with DMSO (control) or indicated concentration of vernodalin for 12 hours. Live cells were stained with DHE dye (green) before cells were fixed and stained with Hoechst 33258 (blue). Images were acquired using Cellomic HCS array scan reader (objective 20 ×). Representative figures (control or 6.25 µg/ml vernodalin-treated) were shown. (B) Bar chart showing average fluorescence intensities of DHE dye in the nucleus. Data were mean ± SD of fluorescence intensity readings representative of three independent experiments. (*P<0.05).