Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1
Figure 5
Co-expression of ALDH3A1 with molecular chaperones.
(A) Co-expression of pG-KJE8/pGro7 plasmid’s chaperones, (B) Co-expression of pTf16 plasmid’s chaperones, (C) Co-expression of pKJE7/pG-Tf2 plasmid’s chaperones. Samples were subjected to SDS-PAGE and stained with Coomassie blue. T0: Total cell extract form bacterial culture prior of protein induction, T3: Total cell extract 3 hours after induction, CE: Crude extract of lysed cells 3 hours after induction, IM: Insoluble matter of lysed cells 3 hours after induction. The recombinant proteins expression is presented in the boxes.