Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1
Figure 2
ALDH3A1 heterologous expression through the pMAL-c2X expression system.
SDS-PAGE pattern showing induction of ALDH3A1 expression at (A) 37°C and (B) 25°C. Samples were subjected to SDS-PAGE and stained with Coomassie blue. Lane 1, whole cell lysate prior IPTG induction; Lane 2, whole cell lysate 6 hours after IPTG induction; Lane 3, insoluble fraction; Lane 4: soluble fraction, respectively, 6 hours after IPTG induction. The arrow indicates the position of ALDH3A1/MBP protein.