Regulation of Wheat Seed Dormancy by After-Ripening Is Mediated by Specific Transcriptional Switches That Induce Changes in Seed Hormone Metabolism and Signaling
Figure 5
Comparison of the transcript abundance of gibberellin (GA) metabolic genes.
The GA metabolism pathway in plants (A). Expression of probesets annotated GA metabolic genes in log2 fold change during imbibition of dormant (D-12/D-0, D-24/D-0 and D-24/D-0) and after-ripened (AR-12/AR-0, AR-24/AR-0 and AR-24/AR-0) seeds as shown in the first column of the heat map, between dormant and after-ripened seeds in both dry and imbibed states (AR-0/D-0, AR-12/D-12 and AR-24/D-24) and between water and ABA imbibed after-ripened seeds (AR-24/AR-24+ABA) as shown in the second column in each heat map (B). Determination of the fold changes in expression of each probeset is as described in Figure 2. Log2 and linear scaled fold changes in expression of the probesets and the respective P values can be found in Table S2. Relative transcript level of TaGA3ox2 in D-0, D-12 and D-24, and AR-0, AR-12 AR-24 and AR-24+ABA wheat seeds (C). Transcript level was determined using Taβactin as the reference gene, and then expressed relative to that in D-0 seeds, which was arbitrarily set to a value of 1. Data are means of 2 to 3 independent biological replicates ± SE. Different letters between seed samples within each imbibition time indicate statistically significant difference in transcript abundance at P≤0.05. GGDP, geranyl geranyl diphosphate CDP, ent-copalyl diphosphate; CPS, ent-copalyl diphosphate synthase KS, ent-kaurene synthase KO, ent-kaurene oxidase KAO ent-kaurenoic acid oxidase GA20ox, gibberellin 20 oxidase GA3ox, gibberellin 3 oxidase; GA2ox, gibberellin 2 oxidase.