Regulation of Wheat Seed Dormancy by After-Ripening Is Mediated by Specific Transcriptional Switches That Induce Changes in Seed Hormone Metabolism and Signaling
Figure 2
Comparison of the transcript abundance of abscisic acid (ABA) metabolic genes and seed ABA content.
The ABA metabolism pathway in plants (A). Expression of probesets annotated as ABA metabolic genes in log2 fold change during imbibition of dormant (D-12/D-0, D-24/D-0 and D-24/D-0) and after-ripened (AR-12/AR-0, AR-24/AR-0 and AR-24/AR-0) seeds as shown in the first column of the heat map, between dormant and after-ripened seeds in both dry and imbibed states (AR-0/D-0, AR-12/D-12 and AR-24/D-24) and between water and ABA imbibed after-ripened seeds (AR-24/AR-24+ABA) as shown in the second column in each heat map (B). Log2 transformed signal intensities of the respective probesets were extracted from the microarray datasets (see Materials and Methods) and converted to expression values in log2 fold changes (the negative and positive numbers on the bar) shown by the color scale at the top of each heat map; higher and lower expression levels of the respective probesets are represented by red and green colors, respectively. Log2 and linear scaled fold changes in expression of the probesets and the respective P values can be found in Table S2. ABA content of D and AR seeds in dry (0 days after imbibition [HAI]) and imbibed (12 and 24 HAI) states (C). Data are means of ABA measurements from three independent biological replicates ± SE. Different letters between imbibition time points and between seed samples within each imbibition time indicate statistically significant difference in seed ABA level at P≤0.05. ZEP (ABA1), zeaxanthin epoxidase; VED, violaxanthin de-epoxidase; ABA4 (NSY), neoxanthin synthase; NCED, 9-cis-epoxycarotenoid dioxygenase; ABA2, ABA deficient 2 (alcohol dehydrogenase); AAO, abscisic aldehyde oxidase; CYP707A; ABA 8′-hydroxylase.