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Lactobacillus reuteri DSM 17938 Changes the Frequency of Foxp3+ Regulatory T Cells in the Intestine and Mesenteric Lymph Node in Experimental Necrotizing Enterocolitis

Figure 2

Effect of breast milk or formula feeding with or without Lactobacillus reuteri DSM 17938 on the frequency of Foxp3+ T cells in the ileum and mesenteric lymph node (MLN) of rats during DOL 0 and DOL 4.

Newborn rats were fed immediately after birth for 4 days. (a) and (c): Changes of single CD4+Foxp3+ Tregs (%) in the ileum (a) and MLN (c); (b) and (d): changes of double CD4+CD8+Foxp3+ T cells in the ileum (b) and MLN (d). The percentage (%) of cells is represented as the mean ± SE of N = 9 observations (pups) in each DOL of each feeding group (total N = 45 for each feeding group).* Dam-fed +17938 vs. Dam-fed, **p<0.01, ***p<0.001. # Formula vs. Dam-fed, # p<0.05, ## p<0.01. &: Formula-fed +17938 vs. Formula-fed, & p<0.05, && p<0.01.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0056547.g002