A New Insect-Specific Flavivirus from Northern Australia Suppresses Replication of West Nile Virus and Murray Valley Encephalitis Virus in Co-infected Mosquito Cells
Figure 5
In vitro superinfection exclusion assay.
IFA and infectious titre assays showing reduced replication of the flaviviruses MVEV and WNVKUNV in C6/36 cells persistently infected with PCV when compared to mock infected (PCV-negative) cells. The alphavirus RRV replicates equally well in both. A–C Study 1; D–F Study 2. A) Co-staining was performed to detect the primary and secondary infecting viruses. The secondary viruses MVEV and RRV were detected with Alexa Fluor 488-labelled mAbs 4G4 and G8 respectively. The PCV primary infection was detected with the anti-PCV mAb 3D6. D) Dramatically less staining for the secondary infecting viruses WNVKUNV and MVEV was detected with mAb 4G4 in cells that were persistently infected with PCV compared with mock-infected cells. In comparison, RRV grew equally well in PCV- and mock-infected cells, as detected with mAb G8.