Resistance to the Novel Fungicide Pyrimorph in Phytophthora capsici: Risk Assessment and Detection of Point Mutations in CesA3 That Confer Resistance
Figure 4
Structure and site of the mutation in the PcCesA3 gene associated with pyrimorph resistance.
(A) Intron/exon structure of the PcCesA3 gene. Numbers represent the size in base pairs. Point mutations in pyrimorph-resistant mutants (RF>50) and the predicted amino acid substitution in the mutant gene products are indicated. (B) Alignment of partial amino acid sequences of CesA3 in P. capsici (PcCesA3). Hd3, Hd11, Hx18, and Dz21 are wild-type isolates. R3-2, R3-3, and R11-1 are pyrimorph-resistant mutants. Mutations in pyrimorph-resistant mutants of P. capsici are indicated by asterisks.