Dam Methylation Participates in the Regulation of PmrA/PmrB and RcsC/RcsD/RcsB Two Component Regulatory Systems in Salmonella enterica Serovar Enteritidis
Figure 5
Relative expression of wzz, rcsB and pmrA mRNA in pmrA and rcsB mutant by real-time quantitative PCR.
Total mRNA was harvested from cultures of SEΔrcsB, SEΔpmrA and S. Enteritidis wild type #5694 (wild type) grown in LB medium (A,B,C) or grown in low Mg2+, low Mg2++Fe3+ and high Mg2+ (D). The relative amount of wzz mRNA was determined by reverse transcription real-time quantitative PCR and related to mRNA levels in wild type strain #5694 (A,B,C) or in wild type strain #5694 grown in low Mg2+ (D), set as 1. Values are means ± SD of five independent mRNA extractions performed in triplicates. * significant difference p<0.01 with respect to wild type strain #5694 grown in the same media; § significant difference p<0.01 with respect to the same strain grown in pmrA-inducing conditions (low Mg2+ and low Mg2++Fe3+ ); ¥ significant difference p<0.05 with respect to the same strain grown in pmrA-inducing conditions (low Mg2+ and low Mg2++Fe3+).