Dam Methylation Participates in the Regulation of PmrA/PmrB and RcsC/RcsD/RcsB Two Component Regulatory Systems in Salmonella enterica Serovar Enteritidis
Figure 3
Relative expression of pmrA rcsB, and wzz mRNA determined by real-time quantitative PCR.
Total mRNA was harvested from cultures of SEΔdam, S. Enteritidis #5694 (wild type) and complemented strains. The relative mRNA amount was determined by reverse transcription real-time quantitative PCR and related to mRNA levels in wild type strain, set as 1. Values are means ± SD of five independent mRNA extractions performed in triplicates. Plasmid pIZ833 bears the dam gen. * significant difference p<0.01 with respect to wild type strain.