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Biochemical Characterization of Uracil Phosphoribosyltransferase from Mycobacterium tuberculosis

Figure 6

Multiple sequence alignment of amino acid sequences of UPRTs from M. tuberculosis, B. caldolyticus, T. gondii, E. coli and S. solfataricus.

Amino acids for each polypeptide sequence were independently numbered. Identical conserved residues are indicated by stars below the alignment. Residues proposed to be involved in catalysis (ARg102 and Asp198), PRPP substrate binding (ARg77 and Arg102), and (or not) C-terminal glycine (Gly205) are highlighted (MtUPRT numbering). Multiple sequence alignment was carried out using Clustal W2 software (http://www.ebi.ac.uk/Tools/msa/clustalw2/).

Figure 6

doi: https://doi.org/10.1371/journal.pone.0056445.g006