Biochemical Characterization of Uracil Phosphoribosyltransferase from Mycobacterium tuberculosis
Figure 5
Apparent steady-state kinetic parameters.
(A) Initial velocity of MtUPRT (U mg−1) as a function of increasing PRPP concentration in the presence of constant uracil concentration (10 µM). (B) Initial velocity of MtUPRT as a function of increasing uracil concentration in the presence of constant PRPP concentration (100 µM). (C) Initial velocity of MtUPRT as a function of increasing PRPP concentration in the presence of constant concentrations of uracil (10 µM) and GTP (100 µM). (D) Initial velocity of MtUPRT as a function of increasing uracil concentration in the presence of constant concentrations of PRPP (100 µM) and GTP (100 µM).