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Biochemical Characterization of Uracil Phosphoribosyltransferase from Mycobacterium tuberculosis

Figure 2

SDS-PAGE analysis of MtUPRT purification steps.

Lane 1, molecular weight protein marker; lane 2, crude extract; lane 3, sample loaded onto DEAE Sepharose CL6B column; lane 4, sample loaded onto Sephacryl S-200 column; lane 5, sample loaded onto Mono Q column; lane 6, homogeneous recombinant MtUPRT eluted from the Mono Q column.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0056445.g002