Single-Step Selection of Drug Resistant Acinetobacter baylyi ADP1 Mutants Reveals a Functional Redundancy in the Recruitment of Multidrug Efflux Systems
Figure 5
Stability of craA mRNA transcript in wild-type and Cm201 backgrounds.
Wild-type and Cm201 cells were grown to mid-logarithmic phase before transcription was arrested with the addition of 200 µg/ml rifampicin. Culture samples were harvested at time points T = 0, 5, 10 and 20, before RNA was extracted and cDNA was synthesised. qRT-PCR was conducted in duplicate using two biological replicates, and the abundance of the craA transcript relative to the rpoB transcript in transcriptionally arrested cells was determined. R2 values and standard deviation is shown.