Development of a Neutralization Assay for Influenza Virus Using an Endpoint Assessment Based on Quantitative Reverse-Transcription PCR
Figure 5
Correlation between neutralization titers measured by qPCR-MN and ELISA-MN.
Adult human sera (n = 20) were assessed by qPCR-MN (input virus of 1000 TCID50 scored by CPE; endpoint assessment at 6 hours post-infection; +TPCK-trypsin) and ELISA-MN (input virus of 100 TCID50 scored by ELISA; endpoint assessment at 22 hours post-infection; -TPCK-trypsin). Neutralization activity against SI/06 was measured. Each point represents the log2 of the geometric mean titer derived from two experimental replicates. Certain points were nudged along the y-axis direction in order to reveal overlaps.