Calcium Mobilization And Protein Kinase C Activation Downstream Of Protease Activated Receptor 4 (PAR4) Is Negatively Regulated By PAR3 In Mouse Platelets
Figure 4
Western blot analysis of protein kinase C (PKC) substrate phosphorylation in mouse platelets.
The level of PKC substrate phosphorylation on serine residues in response to increasing concentrations of: (A) thrombin (1–100 nM) or (C) AYPGKF (0.03–2 mM) was determined by western blotting with phospho-(Ser) PKC substrate antibody. The membranes were re-probed for α-actinin to demonstrate protein loading. The blots shown are from a representative of three independent experiments. Quantitation of PKC substrate phosphorylation in response to (B) thrombin or (D) AYPGKF is represented at the mean (± SD) (* p<0.05).