Reversal of MRP7 (ABCC10)-Mediated Multidrug Resistance by Tariquidar
Figure 3
Effect of tariquidar treatment on protein and mRNA expression of MRP7 in HEK/pcDNA and HEK/MRP7 cells.
(A) The effect of 0.3 µM tariquidar treatment on the protein expression of MRP7 in HEK/pcDNA and HEK/MRP7 cells at 0, 4, 12, 24, 48 and 72 h. (B) The effect of 72 h tariquidar treatment at difference concentrations on the protein expression of MRP7 in HEK/pcDNA and HEK/MRP7 cells. (C) mRNA levels of MRP7 in HEK/pcDNA and HEK/MRP7 cells treated with 0.3 µM tariquidar at different time points. GAPDH was used as a loading control. The protein or mRNA levels of MDR7 were normalized to those of GAPDH and shown on the right. Results are represented as mean ± SD. P values were obtained using analysis of variance by comparing the relative amounts of protein or mRNA in cells treated with tariquidar with those in untreated control cells. Results representative of 3 independent experiments are shown. *: P<0.05; **: P<0.01.