Identification of a Novel Jasmonate-Responsive Element in the AtJMT Promoter and Its Binding Protein for AtJMT Repression
Figure 7
The DNA binding domain of AtBBD1 resides in the C-terminal region.
(A) A schematic representation of truncation mutants of AtBBD1. Numbers indicates amino acid residues, and putative domains are represented (HCR, Highly Conserved Region; DUF151, Domain Unknown Function 151; UVR, putative UV-Response domain) [50]. Each truncated protein was fused with AD as shown in Fig. 5A. PNTR1 (Figure 6) was used as a bait DNA sequence (bottom). (B) Electrophoretic mobility shift assays were carried out using fusion protein (MBP-BBD1) and a 70 bp fragment containing JARE was used as a probe.