Suppression of Hepcidin Expression and Iron Overload Mediate Salmonella Susceptibility in Ankyrin 1 ENU-Induced Mutant
Figure 8
Characterization of Ank1+/Ity16 heterozygous mice.
RBC counts (A) and bacterial burden in spleen, liver and kidney (D) were measured in 7 week old Ank1+/+ wild type and Ank1+/Ity16 heterozygous mice at day 0 (prior infection), day 2 and day 6 post infection. Wild type Ank1+/+ mice are represented by clear circles and Ank1+/Ity16 heterozygous mice by gray circles. Relative kidney mRNA levels are shown for Hamp (C), Il1 (E), IL6 (F) and Epo (G) at day 0, day 2 and day 6 post infection. (B) Relative liver Hamp levels at day 0, day 2 and day 6 post infection. Wild type Ank1+/+ mice are represented by clear bars (n = 3 for day 0 and day 2; n = 4 for day 6) and Ank1+/Ity16 heterozygous mice by gray bars (n = 3 for day 0 and day 2; n = 4 for day 6). (H) Survival curves of Ank1+/+ wild type (n = 5; open circle) and Ank1+/Ity16 heterozygous (n = 5; gray circle) mice with (n = 5; dashed or dotted line) or without (n = 5; solid line) HAMP treatment infected with 5000 CFUs of Salmonella Typhimurium. Log-Rank (Mantel-Cox) for Ank1+/Ity16 heterozygous with and without HAMP treatment p = 0.008. The experiment was repeated twice. An * represents a P-value of less than 0.05; ** represents a P-value of less than 0.001; *** represents a P-value of less than 0.0001.