Inhibitors of the Influenza A Virus M2 Proton Channel Discovered Using a High-Throughput Yeast Growth Restoration Assay
Figure 3
(A) Yeast expressing WT M2 were exposed to 1,440 screening chemicals (M2+ Drugs) with 8 untreated wells per plate as negative controls (M2, n = 144). Each plate also contained 8 positive controls not treated with drugs (Empty plasmid, n = 144). Growth was measured after 40 h and the % growth restoration was calculated as described in Materials and Methods. The arrow points to the single active compound found in this batch. (B) the active compound found in A was retested at different concentrations against yeast expressing WT M2 or bearing the empty plasmid and growth was measured after 40 h.