The Brominated Compound Aeroplysinin-1 Inhibits Proliferation and the Expression of Key Pro- Inflammatory Molecules in Human Endothelial and Monocyte Cells
Figure 5
Aeroplysinin-1 inhibits key processes and the expression levels of key biomolecules in THP-1 human inflammatory monocytes.
A) Aeroplysinin-1 inhibits THP-1 proliferation as determined by the MTT method. B) Aeroplysinin-1 significantly decreases the expression levels of MCP-1 and COX-2 mRNA as determined by qPCR. C) Aeroplysinin-1 significantly decreases the expression levels of COX-2 protein as determined by Western blot. D) Aeroplysinin-1 has no effect on THP-1 migration. E) Aeroplysinin-1 has no effect on THP-1 invasion. Experiments were carried out as described in Material and methods. Data represent mean±SD for three independent experiments (each one with four replicates of each tested concentration).