The Brominated Compound Aeroplysinin-1 Inhibits Proliferation and the Expression of Key Pro- Inflammatory Molecules in Human Endothelial and Monocyte Cells
Figure 4
Aeroplysinin-1 abrogates the expression of PMA-induced COX-2 protein.
A) PMA (50 ng/mL)-induced COX-2 mRNA was detected by qPCR, using the levels of amplification of GAPDH as a control. B) PMA (50 ng/mL)-induced COX-2 protein levels were detected by Western blotting as described, using the levels of β-actin as a control. C) The expression levels of the protein COX-2 were also analyzed in experiments carried out in the presence of cycloheximide (CHX), as shown in a Western blot. Cells were treated with cycloheximide (90 µg/mL) for 1 h. After washing both control and CHX-pretreated cells were treated in complete medium with PMA (50 ng/mL) in the presence or absence of aeroplysinin-1 (10 µM) for 4.5 h. D) The histogram shows the quantification of three independent experiments as that shown in C.