In Vitro Blood-Brain Barrier Models Using Brain Capillary Endothelial Cells Isolated from Neonatal and Adult Rats Retain Age-Related Barrier Properties
Figure 5
The influx rates of drugs in the neonatal and adult rats (in vivo) and the permeability clearance of drugs in the neonatal and adult BBB models (in vitro).
The permeability of drugs (valproic acid (A), nicotine (B), inulin (C)) is expressed as the influx rate in the neonatal (2 weeks, filled columns) and adult (8 weeks, open columns) rats (in vivo: left panels) and the permeability clearance in the neonatal (2 weeks, filled columns) and adult (8 weeks, open columns) BBB models (in vitro: right panels). The influx rate (Kin) was evaluated from the brain tracer uptake in the perfusion period of 120 s and the permeability clearance (PStrans) was evaluated from the transferred volume in the exposure periods of 10, 20, 30 and 40 min. Data are means ± SEM (n = 3−6, in vivo; and n = 4−12 from 2–3 separate experiments, in vitro). * P<0.05, ** P<0.01, significantly different from neonatal rats (in vivo) or neonatal BBB model (in vitro).