Neuroanatomy of the Vestimentiferan Tubeworm Lamellibrachia satsuma Provides Insights into the Evolution of the Polychaete Nervous System
Figure 5
Dorsal nerve cord in L. satsuma.
A, B: Immunoreactivities against anti-acetylated tubulin antibody show that the dorsal nerve cord is continuous from the vestimental region to the trunk. The cord branches in the anterior half of the vestimental region. C–H: Double stain against anti-acetylated tubulin (green) and anti-serotonin (magenta) antibodies shows that the dorsal nerve cord of the trunk is a serotonergic and non-metameric cord. C–E: Confocal laser scanning microscopy of the whole animal. F–H: Cross section of the trunk. I, J: Cross section of the vestimentum. No axon of the dorsal nerve cord was detected by HE staining. K, L: Cross section of the trunk showing the thin fiber of the dorsal nerve cord. Scale bars: A, B = 200 µm; C–H = 100 µm; I–L = 50 µm.