Identification of DVA Interneuron Regulatory Sequences in Caenorhabditis elegans
Figure 2
A. Conserved regions analyzed for DVA expression.
Relative location of conserved regions identified by MUSSA from the acr-15, fax-1, nmr-1 and twk-16 genes. Conserved regions are depicted as red boxes below the corresponding gene and denoted as cs1-cs4 based on their relative position from the first exon in black. The intergenic regions are shown as a black line with the size (kb) above. Neuronal expression is shown in the vertical oriented box as+or - under DVA or Broad. The parentheses and asterisk (53 bp*) following the 73 bp twk-16 cs1 region denotes that the 53 bp fragment of the 73 bp cs1 region expressed in DVA and Broadly. The 73 bp twk-16 cs1 region does not show expression. B. Expression vector. The features of the PCR expression vector are denoted by colors: experimental sequences (red); Δpes-10 (purple); nuclear localization signal (NLS) (blue); YFP (yellow); and derived from the Fire Vector pPD122.53. The unc-119 promoter and unc-119 mini-gene are in green. Experimental sequences were fused by PCR to this expression vector to form a single PCR product.