Adenovirus Serotype 5 Vectors with Tat-PTD Modified Hexon and Serotype 35 Fiber Show Greatly Enhanced Transduction Capacity of Primary Cell Cultures
Figure 5
Ex vivo expansion of CMV pp65-reactive T cells using adenovirus-transduced DCs.
DCs generated from four CMV-seropositive, HLA-A2-positive blood donors were transduced with 100 evg/cell of Ad5PTDf35(pp65) or Ad5(pp65). After expression of the pp65 protein from the viral vector inside the DC the protein is cleaved by proteasomes and the pp65459–503 peptide is presented by HLA-A2 on the surface of the DCs. The pp65-modified DCs were co-cultured with autologous T cells for 11 days to specifically stimulate and expand CMV pp65459–503-reactive CD8+ T cells. The frequency of CMV-pp65-specific T cells (CD3+) were evaluated by HLA-A*0201/pp65459–503 tetramer staining before (pre-stim) and 11 days after stimulation (post-stim).