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Human Trace Amine-Associated Receptor TAAR5 Can Be Activated by Trimethylamine

Figure 5

TMA and DMEA activate hTAAR5 expressed in Xenopus oocytes. A:

IBMX induced currents in oocytes expressing the reporter channel CFTR only. B: In oocytes injected with hTAAR5 cRNA application of TMA and DMEA as well as IBMX as positive control generated currents. C: Test substance induced currents normalized to the corresponding IBMX response. TMA and DMEA induced currents significantly differing from Ringer control (***p<0.001; **p<0.01). Evoked currents were measured by two-electrode voltage clamp technique at a holding potential of −70 mM. Black bars indicate time of agonist application. Error bars represent SEM.

Figure 5

doi: https://doi.org/10.1371/journal.pone.0054950.g005