MicroRNA-195 Inhibits the Proliferation of Human Glioma Cells by Directly Targeting Cyclin D1 and Cyclin E1
Figure 5
miR-195 inhibits glioma proliferation in vivo. A.
Real-time PCR analysis of miR-195 expression in LN18-Vector and LN18-miR-195 stable cell lines. B. Western blotting analysis of Cyclin D1 and Cyclin E1 expression in vector- or miR-195-transduced cells. C. Representative micrographs (left panel) and quantification (right panel) of colonies determined by anchorage-independent growth assays. D–F. Xenograft model in nude mice. Indicated cells were injected into the dorsal flank of the mice. (D), Images of the tumors from all mice. (E), Tumor volumes were measured on the indicated days. (F), Mean tumor weights.