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MicroRNA-195 Inhibits the Proliferation of Human Glioma Cells by Directly Targeting Cyclin D1 and Cyclin E1

Figure 3

Inhibition of miR-195 promotes glioma cell proliferation. A

, MTT assays revealed that inhibition of miR-195 promoted cell growth of glioma cell lines of LN18 and T98G. B, Inhibition of miR-195 promoted the anchorage-independent growth of glioma cells. Representative micrographs (left) and quantification of colonies that were larger than 0.1 mm (right) were scored. C, Representative micrographs (left) and quantification of BrdU incorporating-cells after transfection with NC or miR-195 inhibitor. D, Flow cytometric analysis of indicated glioma cells after transfection with NC or miR-195 inhibitor. Each bar represents the mean of three independent experiments. * P<0.05.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0054932.g003