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A Comparison of Ku0063794, a Dual mTORC1 and mTORC2 Inhibitor, and Temsirolimus in Preclinical Renal Cell Carcinoma Models

Figure 2

Intracellular signaling in RCC cells treated with Ku0063794 or temsirolimus.

Caki-1 (A) and 786-O (B) cells were treated with Ku0063794 at the indicated concentrations for the indicated length of time. Control cells treated with DMSO were treated for 3 hrs. Cell lysates were used for western blotting to analyze the mTORC1/2 pathway. In an analogous experiment, Caki-1 (C) and 786-O (D) cells were similarly treated with temsirolimus or DMSO. The figure is representative of triplicate experiments.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0054918.g002