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A PK2/Bv8/PROK2 Antagonist Suppresses Tumorigenic Processes by Inhibiting Angiogenesis in Glioma and Blocking Myeloid Cell Infiltration in Pancreatic Cancer

Figure 2

PKRA7 decreases subcutaneous pancreatic cancer xenograft tumor growth.

(A) AsPc-1 cells were SC injected into nude mice, and control (n = 4) or PKRA7 (n = 5) treatment was commenced when tumors became visually detectable (9 days). Measurements were taken every 2–3 days. (B) Average tumor weight of control and PKRA7-treated mouse tumors after removal (*p≤0.05). (C) Representative H&E slides from control and PKRA7 treated tumors. (D) Quantification of necrotic regions from 5 slides of each tumor per treatment group, percentages of necrotic areas were measured by ImageJ (p = 0.205719). (E) IHC staining using F4/80 mouse macrophage marker of AsPc-1 SC tumors treated with control or PKRA7. (F) Quantification of average macrophage infiltration of AsPc-1 tumors treated with control (n = 4) or PKRA7 (n = 5), 5 slides of each tumor per treatment group (*p≤0.05).

Figure 2

doi: https://doi.org/10.1371/journal.pone.0054916.g002