Hematopoietic Protein-1 Regulates the Actin Membrane Skeleton and Membrane Stability in Murine Erythrocytes
Figure 2
Loss of Hem1 does not impair erythropoiesis.
Flow cytometric analyses of splenocytes from Hem1−/− vs. wildtype (wt) littermate control mice. (A) Live-gated, Ter119+ cells (left panels) are shown plotted by cell-surface display of CD44 vs. Ter119+ (middle panels) or CD44 vs forward scatter (right panels). Gates defining different erythroid progenitor populations (I-V) are shown. (B) (left) Erythroid progenitor populations are shown as percent of Ter119+ cells. Bars represent mean +/− SEM for three animals per genotype. The asterisks indicate statistical significance: (*), p<0.01; (**), p<0.001. (right) Hem1−/− erythroblasts have normal morphology. Shown are representative photos of WT and Hem1−/− erythroblasts.