Dact2 Represses PITX2 Transcriptional Activation and Cell Proliferation through Wnt/beta-Catenin Signaling during Odontogenesis
Figure 5
Dact2 is a potent inhibitor of Wnt/β-catenin signaling.
293FT cells were transfected with CMV-Lef-1, CMV-PITX2A, CMV-Dact2 overexpression plasmids and TOPFlash reporters. In parallel experiments FOPFlash reporter were transfected as negative control. All luciferase activities were normalized by co-transfected β-galactosidase and ±SEM were from five independent experiments.