Improving Microbial Genome Annotations in an Integrated Database Context
Figure 2
IMG tools for analysis of the correspondence between KO Terms and other protein families in IMG.
(i) The summary table listing all KO terms and their correspondence to other protein families (COGs, Pfams, TIGRfams) based on the genes assigned to both; for instance, KO term K00005 (glycerol dehydrogenase) corresponds to one COG, 2 Pfams and 0 TIGRfams, with 4 unique combinations of all 3 functional assignments. (ii) Detailed lists of genes corresponding to unique combinations of functional assignments using an example of K00005; 4 unique combinations of COG and Pfam for K00005 are shown with the counts of genes assigned to this particular combination, as well as the counts of genes with this combination of COG and Pfam and different KO term assignment or no KO term assignment. In this case the majority of the genes with K00005 term are assigned to COG0371 and pfam01761; however, many genes with the same COG and Pfam are assigned to other KO terms indicating either the lack of consistency of KO term assignment or the broadness of the corresponding COG and Pfam, which may include multiple paralogous proteins with different enzymatic activities.