Integrity and Regeneration of Mechanotransduction Machinery Regulate Aminoglycoside Entry and Sensory Cell Death
Figure 5
BAPTA pre-treatment reduces gentamicin toxicity in hair cells.
A) Cultured cochleae from P3 wildtype mice were treated with BAPTA (5 mM) before gentamicin exposure (0.5 mM×1 hr). After an additional 48 hr AG-free recovery period, tissues were fixed and immunolabeled for myosin7a (green). B–G) Untreated cochlear cultures and cochlear organs treated with BAPTA only did not show hair cell loss. H–J) Gentamicin-treated cultures showed degeneration and a disarrayed arrangement of hair cells. K–M) Pre-treatment with BAPTA led to improved hair cell survival in comparison to treatment with gentamicin alone. N) Quantitative analysis of myosin7a-positive hair cell counts show significantly more hair cells in BAPTA pre-treated organs than those exposed to gentamicin alone. Error bars = S.D., * = p<0.001, scale bar = 25 µm.