Identification and Characterization of Crr1a, a Gene for Resistance to Clubroot Disease (Plasmodiophora brassicae Woronin) in Brassica rapa L.
Figure 5
A. Semi-quantitative RT-PCR analysis of Crr1 in resistant (R4-8-1) and susceptible (A9709) lines. The B. rapa actin gene (BrACT) was used as a control. PCR cycles are indicated on the right. B-D. Spatial expression of Crr1G004 shown as GUS staining in (B) 12-day-old seedlings of Crr1G004 promoter::GUS plants (arrow indicates extreme end of primary root), (C) stele (arrow) of primary root, and (D) cortex (arrow) of primary root. E. Schematic diagram of gene structure of Crr1aG004 and alternative transcripts obtained by RT-PCR using primers flanking introns 2 (RT-a, -b, and -c) and 3 (RT-d and -e). V-like lines, spliced introns; asterisks, predicted stop codons.